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Video Details
Platelet Rich Fibrin (L-PRF) Protocol

Description:
Platelet Rich Fibrin (L-PRF) is an autologous fibrin membrane made by spinning down whole blood and harvesting the platelet and leukocyte containing fibrin fraction. Dr. Maurice Salama’s assistant, Charlene Bennett, will elaborate in detail describing the step-by step PRF preparation and how it can be utilized clinically.

Date Added:
2/7/2012

Author(s):
Charlene Bennett, CDA

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Online Videos / Surgery / Other Surgical / Platelet Rich Fibrin (L-PRF) Protocol




Questions & Comments
Tyler Binner - (4/8/2013 5:03 PM)

Dr. Houenou - according to Dr. Joseph Choukroun (the creator if the PRF Protocol, the fibrin remains stable in the "box" for up to 4 hrs.

Helios Houenou172 - (4/8/2013 10:11 AM)

Thanks for the presentation. Please, tell us how long does the fibrin remain stable before you can no longer use it? A couple of hours? Up to 4 hrs?

Mohammad Sadeghi - (6/30/2012 6:52 AM)

thanks alot ur presentation ur tube has clot activator? we must storeup all tube standup and vertical? i think when carry tube down the good clot in blood and platlat not obtaine

Samuel DeAngelo13 - (3/28/2012 8:42 AM)

Thanks, Kenneth

Kenneth Van Stralen - (3/26/2012 8:59 PM)

I have the PRGF centrifuge and system, and I have the PRF centrifuge and system. I re-programmed my BTI centrifuge to run at the same g force as indicated by the PRF protocol. We took blood from a patient and spun half of it using the BTI centrifuge at the g force indicated by the PRF protcol and half using the PRF centrifuge. The PRF centrifuge produced the fibrin as you see in the above video, the re-programmed PRGF centrifuge did not. Dr. Choukroun has told me that besides the g force (determined by the spin rate x rotor arm length) the angle at which the tubes are spun is critical to achieve the desired result. The US distributor of PRF is Intra Lock.

Samuel DeAngelo13 - (3/26/2012 4:37 PM)

If you own the BTI kit for PRGF, can you utilize different tubes (without anticoagulant) for the fabrication of PRF? Centrifuge the blood for 12 minutes so long as the tube is empty at the start?

Mark Neale - (3/12/2012 8:22 PM)

Thanks, nice prsentation. How long can fibrin stay in the box before you have to use it. Can it dry out or be rendered unusable by taking to much time before using?

William Parker - (3/9/2012 2:37 PM)

How do you compare the technique shown and the growth factors preserved with the much more complex "bti" protocol?? And what company makes the PRF Box??

Arthur Podzhigant - (2/28/2012 5:12 PM)

Nice! Only one question ,which test-tubes do you use?

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