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Video Details
Platelet Rich Fibrin (L-PRF) Protocol

Description:
Platelet Rich Fibrin (L-PRF) is an autologous fibrin membrane made by spinning down whole blood and harvesting the platelet and leukocyte containing fibrin fraction. Dr. Maurice Salama’s assistant, Charlene Bennett, will elaborate in detail describing the step-by step PRF preparation and how it can be utilized clinically.

Date Added:
2/7/2012

Author(s):
Charlene Bennett, CDA
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Questions & Comments
Jason Bressler - (4/15/2016 7:18 PM)

I need some clarity on using PRF membranes for grafting.

After grafting the deficient site with bone, is the next layer over the bone a resorbable or non resorbable membrane AND then the PRF membrane? Or is the the bone, then PRF membrane, and then resorbable or non resorbable?

Any help here would be appreciated!

-Dr Bressler

sameer Thukral - (5/9/2015 6:10 AM)

Dr. Salama.. What is the difference between L-PRF, A-PRF AND I-PRF ? Thanks

Maurice Salama - (3/24/2015 7:14 AM)

Brian; Thanks for being with us in Dallas. Your view is a correct one. There are presentations on this website from both the founders of PRF and PRGF if you want more information about both protocols. Additionally, there are workshops and specific lectures on these topics on this site. Here is one that compares all systems. See link below. regards Dr. Salama http://www.dentalxp.com/Search.aspx?q=PRF

Brian Black - (3/23/2015 11:48 PM)

I just completed the course in Dallas with Dr. Salama. I'm in the process of getting this integrated into my office. After watching both the PRGF and the PRF videos, the PRF seems much simpler to implement in the office. Is there a great benefit to the PRGF over the PRF? From what I see, with the PRF you still get your membrane slug, and liquid for mixing with bone.

George McQueen - (11/10/2014 9:43 AM)

I just attended a workshop in Orlando where they did not cut the fibrin from the rest of the red clot....they simply brushed it off which left just a little red and gave a slightly larger membrane. Thanks for the presentation!!! Also, it was exciting to see how this is being used to help heal ulcers for diabetics etc.

sameh salama - (10/7/2014 7:40 AM)

i would like to inquire about the exact centrifugal force for L-PRF synthesis. in most papers it recommend about 400G however, if i'm not mistaken the process centrifuge in this video is close to the hettich EBA model and taking in prespective of the rotor radius and the 3000 rpm in the demo the centrifugal force is far from 400G. i would like to know if my hypothesis is right or not. thanks

Joseph Choukroun - (7/20/2014 3:43 PM)

Edward, A-PRF will give you more cells than PRF or L-PRF. definitely the presence of these white cells will improve the vasculrization.(synergy of the granulocytes and monocytes). White cells also produce BMP's..
Another interesting observation: the fibrin is less dense and indeed the cell penetration through the fibrin network is faster.. and then the tissue building is faster. Dr Choukroun

edward shapiro - (7/19/2014 8:39 AM)

Charlene. Thanks so much. Seems like a very easy system. I am debating between this and prgf. seems leukocytes are a good thing? How long between drawing blood and spinning. Intralock reps say 1 minute so if drawing 4-8 tubes do you spin for 1 minute first tubes and then spin rest so no coagulation. There are several centrifuges out there for this including Intralock, Dowel and Dr. C himself thru Blusky? Any difference. Also a newer A-prf protocol? is this different. thanks.

Oscar Guzman Sanchez - (6/18/2014 3:45 PM)

Hi, how warm the oven has to be? Also where can I buy these system? Thanks

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