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Video Details
PRGF-Endoret Protocol (Platelet Rich in Growth Factors)

Description:
PRGF®-Endoret Technology is based on the activation of the patient's own platelets for the stimulation and acceleration of tissue healing and regeneration. It is a technology that allows the use of the organism's own resources with extraordinary results in many pathologies, effectively regenerating tissues without side effects and notably reducing the recovery period of fractures, muscular and tendinous injuries, and surgical interventions. Dr. Maurice Salama’s assistant, Charlene Bennett, will elaborate in detail describing the step-by step PRGF preparation and how it can be utilized clinically.

Date Added:
3/3/2013

Author(s):
Charlene Bennett, CDA
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Questions & Comments
Marco Brindis - (4/9/2014 12:05 PM)

I wanted to clarify something. It was mention in this blog that leucocytes can be obtained with the PRF and can not be obtain with the PRGF. The statement is not accurate. With the PRGF system leucocytes can be obtain as well. It was just decided not to use them due to some possible side effects. We could use them if we wanted.

Marco Brindis - (4/9/2014 11:53 AM)

I have been using the PRGF for over 14 years now. The PRGF protocol has been evolved through out the years. I wanted share that the most current protocol is way simpler. Let's start with the Pipets. For many years it was for me kind of a hassle, first, to know when to use the different pipets, second to adjust the quantity on the pipets and finally, and the biggest disadvantage it was to clean the pipets after every patient. The solution with the new protocol is by using a devise that plugs directly to the white empty tube and that will suction the plasma directly in to the tube and the beauty is that is disposable. Because the protocolo has change from dividing the plasma portion in 4 to just 2 portions (The rich in GF and the poor in GF for the fibrin) we don't have to measure anything anymore. It is just half poor half rich. What might seem complicated or confusing in this video is actually the benefit of this system in comparison to the PRF. PRGF is more versatile because it can be mixed with any type of grafting material creating a perfect scaffold to manipulate your grafting procedures. No more particles all over the place. Also you can use the PRGF to bio-activate the surface of the implant for example or even use it to fill up the osteotomy. Concluding with my comment I would like to say that is is wonderful that there are many protocols out there to improve the performance and outcome of our surgeries. PRGF is a versatile one that accommodate to the needs of our department.

Omid Moghaddas - (3/20/2014 1:10 AM)

Larry these are two different preparations by two different concepts. PRF which includes leukocytes and PRGF which does not. the one you first described was PRF and its more user friendly. PRGF preparation according to company instructions (BTI) needs activator to be added which is CaCl and its about 0.5ml per 1ml centrifuged plasma. and thrombin as an anticoagulant is prefilled in the tubes so there is no need to add it.in case if you want to prepare fibrin layer by PRGF system there is no need to add activator. tnx for you question Dr Moghaddas

Larry Burt - (3/19/2014 5:46 PM)

Hello,
So I was at the soft tissue course last weekend and saw this system, but was a little confused in how to use it when compared to what I had been exposed to in the past. Unfortunately after seeing the video I still have some questions - not about what's in the video but the concepts.
First, I have been shown and I have seen other video's that take a fibrin clot in a red tube spun down, pulled out of the tube and cut off the red cells. Then squeeze out most of the plasma in a sterile 2x2 to make a membrane, plug or whatever. I've seen it done just by hand, and other companies have trays to roll out the membrane, or make a plug, etc. No CaCl, thrombin or oven's. Can you help me sort this out.
Also, in the video there seems to be so many fractions and tubes. Is this necessary, a preference or ??? The BTI kit doesn't seem to include the pipetes, extra tubes, etc so is it absolutely necessary or an option. And how do we find out the ratio's/quantities of CaCl and/or thrombin to use, etc.
Thanks for helping me out on this. I really want to do it top drawer but just having a hard time sorting it out.
Larry Burt

Arthur Lyford - (1/25/2014 11:38 AM)

Great job!

mitra mahboob - (9/16/2013 3:27 AM)

Thanks for your great presentation.I don,t understand how the Plasma converted to Fibrin ,because in Hematology generally the Plasma with calcium cholorid convert to fibrin and coaggulate when added a Thrompoblastin agent. My question is do you use other agent that doesn,t mention in movie ?Please tell me tehe answer.thanks .

David Rizk - (8/27/2013 12:50 AM)

Were do we order these supplies from and what are they named or cataloged as. Im a little confused as to what the different layers that you remove from the tube are ? Are you removing these layers from the same yellow colored liquid that is above the red liquid which I assume are the red cells. Maybe you can identify what these layers represent. I have also seen this done centrifuging for 12 mins and the fibrin clot is removed intact from the vial instead of using an oven. How does this differ from your protocol. Dr Rizk

Maurice Salama - (8/21/2013 6:58 AM)

Feng; Is that all you focused upon? It was a depmonstration on the protocol for procuring PRGF. Any relevant questions related to the protocol? Dr. Salama

Feng-Hsiao Hsueh - (8/19/2013 4:05 AM)

how about antiseptic procedure

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